Which Column is Used in HPLC? C18, C8, Ion Exchange & More Explained
Which Column Is Used in HPLC? A Guide to Choosing the Right One In High-Performance Liquid Chromatography (HPLC) , the column is one of the most essential components, responsible for separating the analytes in a sample. The type of column used depends on the chemical nature of the compounds you're analyzing. In this guide, we'll explain the most commonly used HPLC columns and help you choose the right one for your needs. What is an HPLC Column? An HPLC column is a cylindrical tube filled with a stationary phase (usually silica or polymer beads) that interacts with the sample as it moves through the column under high pressure. The mobile phase (liquid solvent) carries the sample through the column, and separation occurs based on the different interactions of each compound with the stationary phase. The type of stationary phase determines the type of column, and this, in turn, affects how the sample components will separate. Types of Columns Used in HPLC 1. C18 Column...